The activities to be carried on will be:

1. Establishment and development of the Iberian Network of laboratories of biological alert.

2. Molecular methods for detection and identification of biological agents will be standardized by developing standard operating procedures (SOPs), thus contributing to harmonization of methods and results. A quality assurance system for the management of the equipment for environmental sampling will be established. The methodology to achieve the defined objectives will be:

  • Accreditation through ISO 15189 of RT PCR methods for detection of:
    • Threats to humans: Yersinia pestis, Clostridium botulinum, Filovirus, Variola virus, Orthopoxvirus, Influenza A, Histoplasma capsulatum, Coccidiodes immitis, Brucella spp., and Plasmodium falciparum.
  • Accreditation through ISO 17025 of RT PCR methods for detection of:
    • Threats to animals: African Swine Fever virus, African Horse Sickness virus, Brucella spp., Coxiella burnetii, Burkholderia mallei, and Burkholderia pseudomallei.
    • Threats to plants: Candidatus Liberibacter spp. and Xhantomonas citri subsp. citri.
    • Environmental matrices: Bacillus anthracis, Burkholderia mallei, and Burkholderia pseudomallei
  • Acreditation through ISO 17025 of serological technique ELISA-DAS for identification of Plum pox virus.
  • Development of protocols for environmental sampling from relevant matrices and implementation of QCA program.

3. Transference of methods to a system for in-field testing: setting malaria, variola and anthrax tests on GenedriveTM system for point of care analysis.

4. Training and exchange of staff and experts through meetings and seminaries.

5. Workshops for dissemination of results: two workshops will be organized at the end of the project for dissemination of results to the potential new members of the network and for transferring the technology for environmental sampling to both the Spanish and Portuguese Security Forces.